Adenylate cyclase 1 Rabbit Polyclonal Antibody

SKU: orb5674

Description

Adenylate cyclase 1 Rabbit Polyclonal Antibody

Research Area

Cancer Research, Energy Metabolism, Metabolism, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Rat
• Predicted Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human adenylate cyclase type I (751-850/1119aa)
• Target: ADCY1
• Molecular Weight: 123 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

3'5' cyclic AMP synthetase; ADCY1; adenyl cyclase; adenylate cyclase type I; ATP pyrophosphate lyase; Brain adenylate cyclase 1; Ca(2+)/calmodulin activated adenylyl cyclase; EC 4.6.1.1; ADCY1_HUMAN; Adenylate cyclase type 1; ATP pyrophosphate-lyase 1; Adenylate cyclase type I; Adenylyl cyclase 1; Ca(2+)/calmodulin-activated adenylyl cyclase..

Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Adenylate cyclase 1) Polyclonal Antibody, Unconjugated (orb5674) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: A549 Cell (Human) Lysate at 30 ug, Primary: Anti-ADCY1 (orb5674) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 123kD, Observed band size: 123kD.

Sample: MCF-7 Cell (Human) Lysate at 30 ug, Primary: Anti-ADCY1 (orb5674) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 123kD, Observed band size: 123kD.

Quick Database Links

Gene Symbol

ADCY1

UniProt

Q08828