ADCY10 Rabbit Polyclonal Antibody

• Catalog Number: orb4345
• Category: Antibodies
• Description: ADCY10 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Mouse, Porcine, Rabbit
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ADCY10 (1101-1200/1610aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 187 kDa
• Target: ADCY10
• UniProt ID: Q96PN6
• RRID: AB_10939274
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Adenylate cyclase 10; Adenylate cyclase homolog; A
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lane 1: Human U-87 MG cell lysates, Lane 2: Human HepG2 cell lysates, Lane 3: Human Jurkat cell lysates, Primary: Anti-ADCY10 (orb4345) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 187 kDa, Observed band size: 55 kDa.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-ADCY10 Polyclonal Antibody, Unconjugated (orb4345) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei.