ADAR1 Rabbit Polyclonal Antibody

• Catalog Number: orb13237
• Category: Antibodies
• Description: ADAR1 Rabbit Polyclonal Antibody
• Target: ADAR
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human DRADA (1001-1226/1226aa)
• UniProt ID: P55265
• RRID: AB_10753341
• MW: 135 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: 136kDa double stranded RNA binding protein; Adar 1
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ADAR1) Polyclonal Antibody, Unconjugated (orb13237) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Sample: A549 (Human) Cell Lysate at 30 ug, Primary: Anti-ADAR1 (orb13237) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 135 kD, Observed band size: 110 kD.

Sample: HepG2 (Human) Cell Lysate at 30 ug, Primary: Anti-ADAR1 (orb13237) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 135 kD, Observed band size: 110 kD.

Sample: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-ADAR1 (orb13237) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 135 kD, Observed band size: 110 kD.