ADAMTS5 Rabbit Polyclonal Antibody

• Catalog Number: orb5678
• Category: Antibodies
• Description: ADAMTS5 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ADAMTS5
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 102 kDa
• Target: ADAMTS5
• UniProt ID: Q9UNA0
• RRID: AB_10925057
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: A disintegrin and metalloproteinase with thrombosp
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lane 1: Mouse Placenta tissue lysates, Lane 2: Mouse Spleen tissue lysates, Lane 3: Human U87MG cell lysates, Lane 4: Human U251 cell lysates, Lane 5: Human SH-SY5Y cell lysates, Lane 6: Human HeLa cell lysates, Primary: Anti-ADAMTS5 (orb5678) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 102 kDa, Observed band size: 102 kDa.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Aggrecanase-2/ADAMTS5 Polyclonal Antibody, Unconjugated (orb5678) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.