ADAMTS5 Rabbit Polyclonal Antibody

SKU: orb5678

Description

ADAMTS5 Rabbit Polyclonal Antibody

Research Area

ADAM Protein Family, Signal Transduction

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: WB=1:500-2000, ELISA=1:5000-10000
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ADAMTS5
• Target: ADAMTS5
• Molecular Weight: 102 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

A disintegrin and metalloproteinase with thrombospondin motifs 5; A disintegrin like and metalloprotease(reprolysin type) with thrombospondin type 1 motif 5; A Disintigrin And Metalloproteinase with ThromboSpondin motif-5; ADAM metallopeptidase with thrombospondin type 1 motif 5; ADAM TS 11; ADAM TS 5; ADAM TS5; ADAMTS 11; ADAMTS 5; ADAMTS11; ADMP 2; ADMP2; Aggrecanase 2; aggrecanase-2; FLJ36738; Implantin; ThromboSpondin motif-5.

Sample: Lane 1: Mouse Placenta tissue lysates, Lane 2: Mouse Spleen tissue lysates, Lane 3: Human U87MG cell lysates, Lane 4: Human U251 cell lysates, Lane 5: Human SH-SY5Y cell lysates, Lane 6: Human HeLa cell lysates, Primary: Anti-ADAMTS5 (orb5678) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 102 kDa, Observed band size: 102 kDa.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Aggrecanase-2/ADAMTS5 Polyclonal Antibody, Unconjugated (orb5678) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

ADAMTS5

UniProt

Q9UNA0