ADAM8 Rabbit Polyclonal Antibody

SKU: orb4376

Description

ADAM8 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, ICC
• Dilution range: ICC/IF=1:100-500, Flow-Cyt=1μg/Test
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ADAM8 52-91aa (51-150/824aa)
• Target: ADAM8
• Molecular Weight: 87 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

A Disintegrin And Metalloproteinase domain 8; A Disintegrin And Metalloproteinase domain 8; ADAM 8; ADAM 8 precursor; ADAM 8 precursor; ADAM metallopeptidase domain 8; ADAM8 protein; CD 156; CD156; CD156a; CD156a antigen; CD156a antigen; Cell surface antigen MS2; Cell surface antigen MS2; Human leukocyte differentiation antigen; ADAM8_HUMAN; Human leukocyte differentiation antigen; Macrophage cysteine rich glycoprotein; Macrophage cysteine rich glycoprotein; MGC134985; MS 2; MS2.

Black line: Positive blank control A431, Negative blank control (HepG2), Green line: Primary Antibody (Rabbit Anti-ADAM8 antibody (orb4376)), Orange line: Isotype Control Antibody (Rabbit IgG). Blue line: Secondary Antibody (Goat anti-rabbit IgG-AF488), A431 (Positive) and HepG2 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ADAM8 Antibody (orb4376) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody (blue) incubation for 40 min at room temperature. Acquisitions of 20000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Blank control: A431. Primary Antibody (green line): Rabbit Anti-ADAM8 antibody (orb4376), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Blank control: Mouse splenocytes (blue), Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), dilution: 1:100 in 1X PBS containing 0.5% BSA, Primary Antibody, dilution: 1 µl in 100 µl 1X PBS containing 0.5% BSA (green).

Quick Database Links

Gene Symbol

ADAM8

UniProt

P78325

References

Conrad, Catharina et al. ADAM8 signaling drives neutrophil migration and ARDS severity JCI Insight, 7, e149870 (2022)

PubMed: 35132956

Applications

IHC

Reactivity

Mouse

Chen, Jun et al. A novel peptide ADAM8 inhibitor attenuates bronchial hyperresponsiveness and Th2 cytokine mediated inflammation of murine asthmatic models Sci Rep, 6, 30451 (2016)

PubMed: 27458083

Applications

IHC

Reactivity

Mouse

Schlomann, Uwe et al. ADAM8 as a drug target in pancreatic cancer Nat Commun, 6, 6175 (2015)

PubMed: 25629724

Applications

IHC, WB

Reactivity

Human