ADAM28 Rabbit Polyclonal Antibody

• Catalog Number: orb100876
• Category: Antibodies
• Description: ADAM28 Rabbit Polyclonal Antibody
• Target: ADAM28
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Mouse
• Predicted Reactivity: Human, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human ADAM28 (321-420/775aa)
• UniProt ID: Q9UKQ2
• MW: 87 kDa
• Tested applications: FC
• Dilution range: Flow-Cyt=1μg/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: A disintegrin and metalloproteinase domain 28; ADA
• Research Area: ADAM Protein Family, ADAMs Protein Family, Cancer
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Zhang, Yu et al. CD19 regulates ADAM28-mediated Notch2 cleavage to control the differentiation of marginal zone precursors to MZ B cells J Cell Mol Med, 21, 3658-3669 (2017)

PubMed: 28707394

Blank control (blue line): mouse blood (fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-ADAM28 antibody (orb100876), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 µg/test.

Blank control (blue line): mouse stomach (fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-ADAM28 antibody (orb100876), Dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1 µg/test.