ADAM17 Antibody

SKU: orb1248027

Description

ADAM17 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 100kDa band observed in Human Testis lysates and approx 125kDa in lysates of cell lines Daudi, HeLa and Jurkat (calculated MW of 93.0kDa according to NP_003174.3). The observed molecular weights correspond to earlier findings in literature with different antibodies (Le Gall et al, J Cell Sci. 2010 Nov 15;123(Pt22):3913-22; PMID: 20980382). Recommended concentration: 0.1-0.3µg/mL. Primary incubated for 1 hour. Preliminary testing was unsuccessful on Mouse and Rat for this particular batch.Immunohistochemistry:Paraffin embedded Human Skin and Thyroid. Recommended concentration: 3.75µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: LQRQNRVDSKETEC
• Target: ADAM17
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ADAM17, TACE, a disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme), CSVP, cSVP, CD156b, CD156b, MGC71942, TACE, cSVPADAM metallopeptidase domain 17, TNF-alpha converting enzyme, a disintegrin and metalloproteinase domain 17, a disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme), snake venom-like protease, ADAM metallopeptidase domain 17, ADAM18, CD156B, ADAM metallopeptidase domain 18, snake venom-like protease, tumor necrosis factor, alpha, converting enzyme

orb1248027 staining (0.2 ug/ml) of Human Testis lysate (RIPA buffer, 35 ug total protein per lane). Detected by chemiluminescence.

orb1248027 staining (0.3 ug/ml) of Daudi (A), Jurkat (B) and HeLa (C) lysates (35 ug total protein in RIPA buffer). Detected by chemiluminescence.

orb1248027 (3.75 ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

ADAM17

UniProt

P78536

RefSeq (Protein):NP_003174.3