ADAM17 Antibody

• Catalog Number: orb1248027
• Category: Antibodies
• Description: ADAM17 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: LQRQNRVDSKETEC
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 100kDa band observed in Human Testis lysates and approx 125kDa in lysates of cell lines Daudi, HeLa and Jurkat (calculated MW of 93.0kDa according to NP_003174.3). The observed molecular weights correspond to earlier findings in literature with different antibodies (Le Gall et al, J Cell Sci. 2010 Nov 15;123(Pt22):3913-22; PMID: 20980382). Recommended concentration: 0.1-0.3ug/ml. Primary incubated for 1 hour. Preliminary testing was unsuccessful on Mouse and Rat for this particular batch.Immunohistochemistry:Paraffin embedded Human Skin and Thyroid. Recommended concentration: 3.75ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ADAM17
• UniProt ID: P78536
• NCBI: NP_003174.3
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: ADAM17, TACE, a disintegrin and metalloproteinase
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 100kDa band observed in Human Testis lysates and approx 125kDa in lysates of cell lines Daudi, HeLa and Jurkat (calculated MW of 93.0kDa according to NP_003174.3). The observed molecular weights correspond to earlier findings in literature with different antibodies (Le Gall et al, J Cell Sci. 2010 Nov 15;123(Pt22):3913-22; PMID: 20980382). Recommended concentration: 0.1-0.3ug/ml. Primary incubated for 1 hour. Preliminary testing was unsuccessful on Mouse and Rat for this particular batch.Immunohistochemistry:Paraffin embedded Human Skin and Thyroid. Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248027 staining (0.2 ug/ml) of Human Testis lysate (RIPA buffer, 35 ug total protein per lane). Detected by chemiluminescence.

orb1248027 staining (0.3 ug/ml) of Daudi (A), Jurkat (B) and HeLa (C) lysates (35 ug total protein in RIPA buffer). Detected by chemiluminescence.

orb1248027 (3.75 ug/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.