ADA Antibody

• Catalog Number: orb371648
• Category: Antibodies
• Description: ADA Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human ADA recombinant protein (Position: Q135-L363). Human ADA shares 82.5% and 82.9% amino acid (aa) sequence identity with mouse and rat ADA, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 40764 MW
• UniProt ID: P00813
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Adenosine deaminase;3.5.4.4;Adenosine aminohydrola
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of Jurkat cells using anti-ADA antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of ADA/Adenosine Deaminase using anti-ADA/Adenosine Deaminase antibody.Lane 1:HELA cell;2:COLO320 cell.

IF analysis of ADA using anti-ADA antibody. ADA was detected in immunocytochemical section of U20S cells.

IHC analysis of ADA/Adenosine Deaminase using anti-ADA/Adenosine Deaminase antibody.ADA/Adenosine Deaminase was detected in paraffin-embedded section of human tonsil tissues.