ACY1 Antibody

SKU: orb1260412

Description

ACY1 Antibody

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-408 of human ACY1 (NP_001185824.1).
• Target: ACY1
• Molecular Weight: Observed: 46kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

ACY1, Aminoacylase-1, ACY-1, ACY1D, Acylase, Acylase I, Aminoacylase 1, Aminoacylase, Aminoacylase I

Western blot analysis of extracts of various cell lines, using ACY1 antibody (orb1260412) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded rat kidney using ACY1 antibody (orb1260412) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human esophageal cancer using ACY1 antibody (orb1260412) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of MCF7 cells using ACY1 antibody (orb1260412). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

ACY1

UniProt

Q03154