ACTN3 antibody

• Catalog Number: orb390204
• Category: Antibodies
• Description: Rabbit polyclonal antibody to ACTN3.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 580-630 of human ACTN3 (NP_001095.2).
• Dilution range: WB: 1:500-2000
• Conjugation: Unconjugated
• MW: 103kDa
• UniProt ID: Q08043
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
• Alternative names: ACTN3D; ACTN3
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry analysis of paraffin-embedded mouse skeletal muscle using ACTN3 Rabbit pAb (orb390204) at dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded rat skeletal muscle using ACTN3 Rabbit pAb (orb390204) at dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Western blot analysis of various lysates using ACTN3 Rabbit pAb (orb390204) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.