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Catalog Number | orb381028 |
---|---|
Category | Antibodies |
Description | ACTN3 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human ACTN3 (574-617aa EADRERGAIMGIQGEIQKICQTYGLRPCSTNPYITLSPQDINT K), different from the related mouse sequence by five amino acids. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Western blot, 0.1-0.5μg/ml, Human, Mouse, RatFlow Cytometry, 1-3μg/1x106 cells, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 103241 MW |
UniProt ID | Q08043 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Alpha-actinin-3;Alpha-actinin skeletal muscle isof Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of WISH cells using anti-ACTN3 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of ACTN3/Alpha Actinin 3 using anti-ACTN3/Alpha Actinin 3 antibody.Lane 1:human HT1080 cell; 2:rat skeletal muscle tissue; 3:rat skeletal muscle tissue; 4:mouse skeletal muscle tissue; 5:mouse skeletal muscle tissue.
IHC analysis of ACTN3/Alpha Actinin 3 using anti-ACTN3/Alpha Actinin 3 antibody.ACTN3/Alpha Actinin 3 was detected in paraffin-embedded section of mouse skeletal muscle tissues.
IHC analysis of ACTN3/Alpha Actinin 3 using anti-ACTN3/Alpha Actinin 3 antibody.ACTN3/Alpha Actinin 3 was detected in paraffin-embedded section of rat skeletal muscle tissues.
IHC analysis of ACTN3/Alpha Actinin 3 using anti-ACTN3/Alpha Actinin 3 antibody.ACTN3/Alpha Actinin 3 was detected in paraffin-embedded section of human lung cancer tissues.
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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