ACTN2 Rabbit Polyclonal Antibody

SKU: orb578075

Description

Rabbit polyclonal antibody to ACTN2

Research Area

Cell Biology, Disease Biomarkers, Molecular Biology, Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 4

Tested Applications	IHC, WB
Reactivity	Human
Predicted Reactivity	Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat

Related Conjugates & Formulations

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Biotin

FITC

HRP

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	The immunogen is a synthetic peptide directed towards the N terminal region of human ACTN2
Target	ACTN2
Protein Sequence	Synthetic peptide located within the following region: NQIEPGVQYNYVYDEDEYMIQEEEWDRDLLLDPAWEKQQRKTFTAWCNSH
Molecular Weight	104 kDa
Purification	Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration	0.5 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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MPD6, CMH23, CMD1AA, MYOCOZ

Sarcomeric Alpha Actinin Rabbit Polyclonal Antibody [orb158338]

Applications:	IF, IHC-Fr, IHC-P
Predicted Reactivity:	Bovine, Canine, Equine, Gallus, Human, Porcine, Rabbit, Sheep
Reactivity:	Mouse, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Sizes Available: 50 μl, 100 μl, 200 μl

Item 1 of 5
ACTN2 Rabbit Polyclonal Antibody [orb763156]
Applications:
ELISA, FC, IHC, WB
Reactivity:
Human, Mouse, Rat
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 100 μg
Item 1 of 4
ACTN2 Rabbit Polyclonal Antibody [orb578076]
Applications:
IHC, WB
Predicted Reactivity:
Bovine, Canine, Equine, Guinea pig, Mouse, Rabbit, Rat, Zebrafish
Reactivity:
Human
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Unconjugated
Sizes Available: 100 μl

Quality Guarantee

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25 ug of the indicated Human whole cell extracts was loaded onto a 6-18% SDS-PAGE gel. 0.2 ug/ml of the antibody was used in this experiment. A smaller isoform is visible at 68 kDa in some samples.

Lanes: Lane 1: 10 ug ACTN1-GFP transfected COS-7 lysate, Lane 2: 10 ug ACTN2-GFP transfected COS-7 lysate, Lane 3: 10 ug ACTN3-GFP transfected COS-7 lysate, Lane 4: 10 ug ACTN4-GFP transfected COS-7 lysate, Primary Antibody Dilution: 1:1000, Secondary Antibody: Anti-rabbit HRP, Secondary Antibody Dilution: 1:5000, Gene Name: ACTN2.

Sample Type: ACTNX-GFP transfected COS-7 cells, Primary Antibody Dilution: 1:1000, Secondary Antibody: Anti-rabbit-Alexa-Fluor 568, Secondary Antibody Dilution: 1:100, Color/Signal Descriptions: Green: GFP Red: ACTN2, Gene Name: ACTN2.

WB Suggested Anti-ACTN2 Antibody Titration: 0.2-1 ug/ml, ELISA Titer: 1:312500, Positive Control: Human Muscle.

Quick Database Links

Gene Symbol

ACTN2

UniProt

P35609

RefSeq (Protein):NP_001094

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_001094

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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