ACTG1 Antibody

• Catalog Number: orb1263815
• Category: Antibodies
• Description: ACTG1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, WB
• Predicted Reactivity: Bovine, C. elegans, Drosophila, Gallus, Hamster, Monkey, Mouse, Porcine, Rabbit, Rat, Xenopus, Yeast, Zebrafish
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This ACTG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 188-215 amino acids from the Central region of human ACTG1.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 42 kDa
• Target: ACTG1
• UniProt ID: P63261
• NCBI: P63261
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Actin, cytoplasmic 2, Gamma-actin, Actin, cytoplas
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ACTG1 Antibody in K562, HepG2 cell line lysates (35 ug/lane)

Flow cytometric analysis of K562 cells using ACTG1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of ACTG1 using rabbit polyclonal ACTG1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ACTG1 gene.

Confocal immunofluorescent analysis of ACTG1 Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).