ACPT Rabbit Polyclonal Antibody

• Catalog Number: orb101887
• Category: Antibodies
• Description: ACPT Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC
• Predicted Reactivity: Canine, Equine, Mouse, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ACPT (65-160/437aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: Flow-Cyt=0.2ug/test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 47 kDa
• Target: ACP4
• UniProt ID: Q9BZG2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Acid phosphatase testicular; Testicular acid phosp
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Liang, Tian et al. Enamel defects in Acp4R110C/R110C mice and human ACP4 mutations Sci Rep, 12, 16477 (2022)

PubMed: 36183038

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-ACPT antibody (orb101887), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, Dilution: 0.2 µg/test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.