ACPT Rabbit Polyclonal Antibody

SKU: orb101887

Description

ACPT Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC
• Dilution range: Flow-Cyt=0.2ug/test
• Reactivity: Human
• Predicted Reactivity: Canine, Equine, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ACPT (65-160/437aa)
• Target: ACP4
• Molecular Weight: 47 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Acid phosphatase testicular; Testicular acid phosphatase; PPAT_HUMAN.

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-ACPT antibody (orb101887), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, Dilution: 0.2 µg/test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

ACP4

UniProt

Q9BZG2

References

Brent Preston Vasquez The Impact of Amelogenin Phosphorylation on Biological Processes Within the Tooth Organ search.proquest.com, (2025)

PubMed:

Applications

IHC

Reactivity

Mouse

Liang, Tian et al. Enamel defects in Acp4R110C/R110C mice and human ACP4 mutations Sci Rep, 12, 16477 (2022)

PubMed: 36183038