ACPP Antibody

• Catalog Number: orb1249269
• Category: Antibodies
• Description: ACPP Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-NHMKRATQIPSYKK
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 50kDa band observed in Human Prostate lysates (calculated MW of 48.3kDa according to NP_001127666.1). Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:In paraffin embedded Human Prostate shows reticulate cytoplasm staining, more intens towards the lumen of the gland. Recommended concentration, 3-5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ACPP
• UniProt ID: P15309
• NCBI: NP_001090.2, NP_001127666.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: ACPP, acid phosphatase, prostate, ACP-3, ACP3, PAP
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 50kDa band observed in Human Prostate lysates (calculated MW of 48.3kDa according to NP_001127666.1). Recommended concentration: 0.01-0.03ug/ml.Immunohistochemistry:In paraffin embedded Human Prostate shows reticulate cytoplasm staining, more intens towards the lumen of the gland. Recommended concentration, 3-5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1249269 (0.01 ug/ml) staining of Human Prostate lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1249269 (3.8 ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.