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Catalog Number | orb20559 |
---|---|
Category | Antibodies |
Description | Goat polyclonal to ACPP |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC, WB |
Reactivity | Bovine, Canine, Human, Mouse, Rat |
Dilution range | ELISA: 1:64000, WB: 0.3-1 μg/ml, IHC-P: 3-5µg/ml |
Conjugation | Unconjugated |
MW | 44.6 |
Target | ACPP / PAP |
Entrez | 55 |
Protein Sequence | YGIHKQKEKSRLQ |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti ACPP antibody, anti acid phosphatase, prostat Read more... |
Note | For research use only |
Application notes | IHC: In paraffin embedded Human Prostate shows cytoplasm staining in some secretory cells of the gland. Recommended concentration, 3-5μg/ml.WB: Approx 50kDa band observed in Human Prostate lysates (calculated MW of 44.6kDa according to NP_001090.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Patel et al, ,Clin Chem. 1986 Oct;32(10):1832-5. PMID: 3530530). Recommended concentration: 0.3-1 μg/ml. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of human prostate lysate (35µg protein in RIPA buffer) using ACPP antibody
Immunohistochemical analysis of formalin-fixed paraffin embedded human prostate tissue using ACPP antibody
ELISA, IHC | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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