ACP5 Rabbit Polyclonal Antibody

SKU: orb2250

Description

ACP5 Rabbit Polyclonal Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1ug/Test
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine, Equine, Porcine, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human TRAP/Tartrate Resistant Acid Phosphatase (101-200/325aa)
• Target: ACP5
• Molecular Weight: 34 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Type 5 acid phosphatase; Acid phosphatase 5 tartrate resistant; PPA5_HUMAN; T5ap; Tartrate resistant acid ATPase; Tartrate resistant acid phosphatase type 5; Tartrate resistant acid phosphatase type 5 precursor; Tartrate-resistant acid ATPase; Tartrate-resistant acid phosphatase type 5; TR AP; TR-AP; TrATPase; Type 5 acid phosphatase.

Blank control (black line): Hela. Primary Antibody (green line): Rabbit Anti-ACP5 antibody (orb2250), dilution: 1 ug/Test, Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF488, dilution: 0.5 ug/Test. Isotype control (orange line): Normal Rabbit IgG, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Liyer (Mouse) Cell Lysate at 40 ug, Primary: Anti-Tartrate Resistant Acid Phosphatase (orb2250) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 34 kD, Observed band size: 34 kD.

Sample: MOLT-4 (Human) Cell Lysate at 30 ug, Primary: Anti-Tartrate Resistant Acid Phosphatase (orb2250) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 34 kD, Observed band size: 35 kD.

Quick Database Links

Gene Symbol

ACP5

UniProt

P13686

References

Romulo de Oliveira Sales-Junior 1, Bharbara de Moura Pereira 1, Rafaela Ricci 1, Nathália Evelyn da Silva Machado 1, Julissa Denisse Arguello Alvarado 1, Ana Beatriz Carreto 1, Edilson Ervolino 2, Anil Kishen 3, Luciano Tavares Ângelo Cintra 1, João Eduardo Gomes-Filho Systemic administration of polyphenols from dealcoholized red wine reduces inflammation and bone resorption in established apical periodontitis in male rats Arch Oral Biol ., (2025)

PubMed: 39986211

Applications

IHC

Reactivity

Rat

Juliano Milanezi de Almeida 1 2, Halef Diego Turini 1, Henrique Rinaldi Matheus 1 2 3, Otávio Augusto Pacheco Vitória 1 2, Bianca Rafaeli Piovezan 1 2, Ruan Henrique Barra Dalmonica 1 2, Elisa Mara de Abreu Furquim 1 2, Edilson Ervolino Omega-3 attenuates the severity of medication-related osteonecrosis of the jaws in rats treated with zoledronate PLoS One ., (2025)

PubMed: 40138277

Liu, Tong-Jun et al. Overexpression of microRNA-141 inhibits osteoporosis in the jawbones of ovariectomized rats by regulating the Wnt/β-catenin pathway Arch. Oral Biol., 113, 104713 (2020)

PubMed: 32229339