ACL Antibody

• Catalog Number: orb1524033
• Category: Antibodies
• Description: ACL Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 350 and 400
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: ACL
• UniProt ID: P53396
• NCBI: NP_001087.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: ATPCL; CLATP; citrate cleavage enzyme; ATP-citrate
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human ACL by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3 and CT26 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ACL antibody (orb1524033) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human ACL by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, Hep-G2, HEK293T, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ACL antibody orb1524033 (orb1524033) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human ACL by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ACL antibody (orb1524033) used for IP at 6 µg per reaction. ACL was also immunoprecipitated by a previous lot of this antibody (orb1524033) and a second antibody against a different epitope of ACL.