ATP citrate lyase/ACLY Antibody

• Catalog Number: orb381026
• Category: Antibodies
• Description: ATP citrate lyase/ACLY Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human ATP citrate lyase recombinant protein (Position: M1-I180). Human ATP citrate lyase shares 95% amino acid (aa) sequence identity with both mouse and rat ATP citrate lyase.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 120839 MW
• UniProt ID: P53396
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: ATP-citrate synthase;2.3.3.8;ATP-citrate (pro-S-)-
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of HepG2 cells using anti-ACLY antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of ACLY using anti-ACLY antibody.Lane 1:human A549 cell; 2:human MOLT4 cell; 3:human U87 cell; 4:human U251 cell; 5:human 293T cell; 6:human HeLa cell; 7:human T47D cell; 8:human HepG2 cell; 9:rat pancreas tissue; 10:mouse NIH/3T3 cell.

IF analysis of ACLY using anti-ACLY antibody. ACLY was detected in an immunocytochemical section of A594 cells.

IHC analysis of ACLY using anti-ACLY antibody. ACLY was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue.

IHC analysis of ACLY using anti-ACLY antibody. ACLY was detected in a paraffin-embedded section of human hepatocellular carcinoma tissue.

IHC analysis of ACLY using anti-ACLY antibody. ACLY was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue.

IHC analysis of ACLY using anti-ACLY antibody. ACLY was detected in a paraffin-embedded section of human placenta tissue.

IHC analysis of ACLY using anti-ACLY antibody. ACLY was detected in a paraffin-embedded section of human colon cancer tissue.

IHC analysis of ACLY using anti-ACLY antibody. ACLY was detected in a paraffin-embedded section of mouse testis tissue.

IHC analysis of ACLY using anti-ACLY antibody. ACLY was detected in a paraffin-embedded section of rat testis tissue.