Acetyl-HIST1H2BC (K20) Antibody

• Catalog Number: orb517018
• Category: Antibodies
• Description: This Acetyl-HIST1H2BC (K20) Antibody is an unconjugated polyclonal product. It tagets HIST1H2BC using a Peptide sequence around site of Acetyl-Lys (20) derived from Human Histone H2B type 1-C/E/F/G/I as the immunogen. This antibody is suitable for ChIP, ELISA, ICC, IF, IP, WB. Purification: Antigen Affinity Purified.
• Target: HIST1H2BC
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Buffer/Preservatives: Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol
• Purification: Antigen Affinity Purified
• Immunogen: Peptide sequence around site of Acetyl-Lys (20) derived from Human Histone H2B type 1-C/E/F/G/I
• UniProt ID: P62807
• Tested applications: ChIP, ELISA, ICC, IF, IP, WB
• Dilution range: WB:1:500-1:5000, ICC:1:500-1:1000, IF:1:50-1:200, IP:1:200-1:2000
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: H2BC4 antibody; H2BFL antibody; HIST1H2BC; antibod
• Research Area: Epigenetics & Chromatin
• Note: For research use only

Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti- Acetyl-HIST1H2BC (K20) antibody or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real

Western blot aanalysis of Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, K562 whole cell lysateusing Acetyl-HIST1H2BC (K20) antibody

Immunoprecipitation analysis of A549 whole cell lysate using Acetyl-HIST1H2BC (K20) antibody

Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) using Acetyl-HIST1H2BC (K20) antibody

Immunocytochemistry analysis of Hela cells using Acetyl-HIST1H2BC (K20) antibody