ACCN1/ASIC2 Antibody

• Catalog Number: orb623787
• Category: Antibodies
• Description: ACCN1/ASIC2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human ACCN1/ASIC2 recombinant protein (Position: H27-C512).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human Direct ELISA, 0.1-0.5μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 58 kDa
• UniProt ID: Q16515
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Acid-sensing ion channel 2; ASIC2; Amiloride-sensi
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis using anti-ASIC2 antibody.1:placenta tissue, 2:PC-3 cell, 3:A549 cell, 4:U2OS cell, 5:rat brain tissue, 6:rat liver tissue, 7:rat ovarian tissue, 8:mouse brain tissue, 9:mouse liver tissue, 10:mouse ovarian tissue.

IF analysis of ASIC2 using anti-ASIC2 antibody.

Flow Cytometry analysis of A549 cells using anti-ASIC2 antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG.Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of PC-3 cells using anti-ASIC2 antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG.Unlabelled sample (Red line) was also used as a control.