ACCN1 Rabbit Polyclonal Antibody

• Catalog Number: orb1004
• Category: Antibodies
• Description: ACCN1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Canine, Equine, Mouse, Rabbit
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ACCN1 (301-400/512aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 56 kDa
• Target: ASIC2
• UniProt ID: Q16515
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: ACCN; ASIC2a; BNaC1; ASIC2; ASICs2 ACCN1_HUMAN; Ac
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lane 1: Human SH-SY5Y cell lysates, Lane 2: Human U251 cell lysates, Lane 3: Human U-2 OS cell lysates, Lane 4: Human Jurkat cell lysates, Primary: Anti-ACCN1 (orb1004) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56 kDa, Observed band size: 60, 63 kDa.

Tissue/cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-ACCN1 Polyclonal Antibody, Unconjugated (orb1004) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.