ABO Antibody

• Catalog Number: orb1252541
• Category: Antibodies
• Description: ABO Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: HE-193
• Tested applications: FA, IF, IHC-P
• Reactivity: Human
• Isotype: IgM, kappa
• Immunogen: A mixture of erythrocytes of blood group A and glycoprotein fraction isolated from the saliva of secretors with blood group A was used as the immunogen for the ABO antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Functional Activity-Erythrocyte AgglutinationImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Optimal dilution of the ABO antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ABO
• UniProt ID: P16442
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Histo-blood group ABO system transferase, Fucosylg
• Note: For research use only
• Application notes: Functional Activity-Erythrocyte AgglutinationImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Optimal dilution of the ABO antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Expiration Date: 12 months from date of receipt.

Formalin-fixed, paraffin-embedded human colon carcinoma stained with Blood Group Antigen A antibody (HE-193).