ABO Antibody

• Catalog Number: orb1252539
• Category: Antibodies
• Description: ABO Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: HEB-20
• Tested applications: FA, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgM, kappa
• Immunogen: A mixture of erythrocytes of group B and glycoprotein fraction isolated from saliva of secretors with blood group B was used as the immunogen for the ABO antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Functional Activity-Erythrocyte AgglutinationImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the ABO antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ABO
• UniProt ID: P16442
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Histo-blood group ABO system transferase, Fucosylg
• Note: For research use only
• Application notes: Functional Activity-Erythrocyte AgglutinationImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the ABO antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Formalin-fixed, paraffin-embedded human colon carcinoma stained with Blood Group B antibody (HEB-20).

SDS-PAGE Analysis of Purified, BSA-Free ABO Antibody (clone HEB-20). Confirmation of Integrity and Purity of the Antibody.