ABCF2 Rabbit Polyclonal Antibody

• Catalog Number: orb155558
• Category: Antibodies
• Description: ABCF2 Rabbit Polyclonal Antibody
• Target: ABCF2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Porcine
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human ABCF2 (301-400/623aa)
• UniProt ID: Q9UG63
• MW: 71 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: 0710005O05Rik; ABC28; ABCF 2; ATP binding cassette
• Research Area: DNA / RNA Binding, DNA and RNA Research, Signal Tr
• Note: For research use only

25 ug total protein per lane of various lysates probed with ABCF2 polyclonal antibody, unconjugated (orb155558) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ABCF2) Polyclonal Antibody, Unconjugated (orb155558) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.