ABCC9 Rabbit Polyclonal Antibody

SKU: orb330328

Description

Rabbit polyclonal antibody to ABCC9

Research Area

Cell Biology, Molecular Biology

Images & Validation

• Tested Applications: WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Goat, Guinea pig, Rabbit, Rat, Zebrafish

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ABCC9
• Target: ABCC9
• Molecular Weight: 174kDa
• Purification: Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: 0.5 mg/ml
• Disclaimer: For research use only

Alternative Names

anti ABC37 antibody, anti CMD1O antibody, anti FLJ36852 antibody, anti SUR2 antibody, anti ATFB12 antibody

Sample Tissue: Human PANC1, Antibody Dilution: 1.0 ug/mL.

Lanes: 1: 10 ug SUR1 KO mouse ventricle lysate, 2: 10 ug WT mouse ventricle lysate, 3: 0.1 ug SUR1 overexpressing mouse ventricle lysate, 4: 10 ug cannine ventricle lysate, Primary Antibody Dilution: 1:1000, Secondary Antibody: Anti-rabbit HRP, Secondary Antibody Dilution: 1:2000, Gene Name: ABCC9.

Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip (PAGH200M) at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.

WB Suggested Anti-ABCC9 Antibody Titration: 0.2-1 ug/mL, ELISA Titer: 1:1562500, Positive Control: 721_B cell lysate, ABCC9 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.

Quick Database Links

Gene Symbol

ABCC9

UniProt

O60706

RefSeq (Protein):NP_005682