ABCB6 Antibody

SKU: orb345555

Description

ABCB6 antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:40,000 - 1:130,000, WB: 1:1,000 - 1:5,000
• Reactivity: Canine, Human
• Application Notes:
  This affinity-purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 94 kDa in size corresponding to ABCB6 protein by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 430-465 of human ABCB6 protein.
• Purity: This affinity-purified antibody is directed against human ABCB6 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with ABCB6 protein from human and dog based on 100% homology with the immunizing sequence. Expect partial reactivity with ABCB6 from mouse and rat sources based on partial (~93%, 15/16) sequence homology. Reactivity against homologues from other sources is not known.

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.0 mg/ml
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-ABCB6 Antibody, ABCB 6, ABCB-6, ABC transporter umat antibody, ABC14 antibody, ATP binding cassette sub family B member 6, mitochondrial precursor antibody, mitochondrial precursor ABC transporter 3, Mt-ABC transporter 3

Western blot using Biorbyt's affinity purified anti-ABCB6 antibody (right panel, lane B6) shows detection of Flag tagged human ABCB6 protein at 104 kDa and a truncated form of the protein at 79 kDa (arrowheads). The antibody successfully detected ABCB6 in KB cells transfected with the ABCB6 protein. A lysate prepared from KB cells without a vector insert (CV lane) showed no reactivity with the antibody. The left panel shows a similar staining pattern using an anti-Flag™ antibody as a positive control. The membrane was probed with the primary antibody diluted to 1:1400.

Quick Database Links

UniProt

Q9NP58

RefSeq:13123949