ABCB5 Antibody

SKU: orb345620

Description

ABCB5 antibody

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:10,000 - 1:15,000, IHC: 1:200, WB: 1:10,000
• Reactivity: Human
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA, IHC, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 117 kDa in size corresponding to ABCB5 by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues corresponding to an internal region of human ABCB5.
• Target: ABCB5
• Purity: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for human ABCB5 protein. A BLAST analysis was used to suggest partial cross-reactivity with ABCB5 from monkey (85% homology), rat (68% homology) and mouse (62% homology) sources. Cross-reactivity with ABCB5 from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 0.31 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-ABCB5 Antibody, ABCB 5, ABCB-5, ATP binding cassette sub family B (MDR/TAP) member 5 antibody, ATP binding cassette sub family B member 5 antibody, EST422562 antibody, P glycoprotein ABCB5 antibody

Quick Database Links

Gene Symbol

ABCB5

UniProt

Q2M3G0

RefSeq:56849536