ABCB5 Antibody

SKU: orb345619

Description

ABCB5 antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:10,000 - 1:50,000, IHC: 1:200, WB: 1:20,000
• Reactivity: Human
• Application Notes:
  This affinity purified ABCB5 antibody has been tested for use in ELISA, IHC, and western blotting. Specific conditions for reactivity should be optimized by the end user.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: ABCB5 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues corresponding to an internal region of human ABCB5.
• Purity: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for human ABCB5 protein. A BLAST analysis was used to suggest partial cross-reactivity with ABCB5 from monkey (85% homology), rat (68% homology) and mouse (62% homology) sources. Cross-reactivity with ABCB5 from other sources has not been determined.

Storage & Handling

• Storage: Store Anti-ABCB5 at -20° C prior to opening. Aliquot antibody and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.1 mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-ABCB5 Antibody, ABCB 5, ABCB-5, ATP binding cassette sub family B (MDR/TAP) member 5 antibody, ATP binding cassette sub family B member 5 antibody, EST422562 antibody, P glycoprotein ABCB5 antibody

Immunohistochemistry of ABCB5 antibody. Tissue: human liver. Fixation: formalin fixed paraffin embedded. Antigen retrieval: user optimized. Primary antibody: ABCB5 at 1:200. Secondary antibody: Peroxidase goat anti-rabbit at 1:10000 for 45 min at RT. Localization: Moderate to strong cytoplasmic and membranous staining was observed in hepatocytes. Occasional nuclear staining was observed in hepatocytes and sinusoidal cells. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.

Immunohistochemistry of Rabbit anti ABCB5 antibody. Tissue: human breast carcinoma at pH6. (left, neg control) (right, 20X, 40X). Fixation: formalin fixed paraffin embedded. Primary antibody: ABCB5 antibody at 10 µg/ml for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10000 for 45 min at RT. Localization: ABCB5 is cytoplasmic. Staining: ABCB5 as precipitated brown signal with hematoxylin purple nuclear counterstain.

Immunohistochemistry of Rabbit anti ABCB5 antibody. Tissue: human breast carcinoma at pH9. (20X, 40X) Fixation: formalin fixed paraffin embedded. Primary antibody: ABCB5 antibody at 10 µg/ml for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10000 for 45 min at RT. Localization: ABCB5 is cytoplasmic. Staining: ABCB5 as precipitated brown signal.

Western blot using Biorbyt's affinity purified anti-ABCB5 antibody shows detection of ABCB5 beta in ~12.5 ug of transfected-Hi5 whole cell lysate. No reaction was seen when antibody was pre-incubated with the immunizing peptide (data not shown). A 3-8% Tris-acetate gel was used for separation. The arrowhead corresponds to 117 kDa ABCB5. The membrane was probed with the primary antibody at a 1:10000 dilution in 5% milk in TBST at 4°C, overnight.

Quick Database Links

UniProt

Q2M3G0

RefSeq:56849536