ABCA7 Rabbit Polyclonal Antibody

SKU: orb1068

Description

ABCA7 Rabbit Polyclonal Antibody

Research Area

ATPases, Astrocyte Markers, Cell Biology, Cholesterol Metabolism, Lipid Metabolism, Metabolism, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: FC
• Dilution range: Flow-Cyt=1ug/test
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ABCA7 (251-350/2146aa)
• Target: ABCA7
• Molecular Weight: 234 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

ABC transporter ABCA7; ABC transporter member 7; ABCA SSN; ABCX; ATP binding cassette sub family A(ABC1) member 7; ATP binding cassette sub family A member 7; ATP binding Cassette Transporter A7; , FLJ40025; Macrophage ABC transporter; ABCA7_HUMAN.

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-ABCA7 antibody (orb1068), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 20% PBST for 20 min atroom temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ABCA7) Polyclonal Antibody, Unconjugated (orb1068) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

ABCA7

UniProt

Q8IZY2