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ABCA7 Rabbit Polyclonal Antibody

Catalog Number: orb1068

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb1068
CategoryAntibodies
DescriptionABCA7 Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC
Predicted ReactivityMouse, Rat
ReactivityHuman
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human ABCA7 (251-350/2146aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeFlow-Cyt=1ug/test
Form/AppearanceLiquid
ConjugationUnconjugated
MW234 kDa
TargetABCA7
UniProt IDQ8IZY2
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesABC transporter ABCA7; ABC transporter member 7; A
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NoteFor research use only
Expiration Date12 months from date of receipt.
ABCA7 Rabbit Polyclonal Antibody

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-ABCA7 antibody (orb1068), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 20% PBST for 20 min atroom temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

ABCA7 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ABCA7) Polyclonal Antibody, Unconjugated (orb1068) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

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