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ABCA7 Rabbit Polyclonal Antibody

Catalog Number: orb1068

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb1068
CategoryAntibodies
DescriptionABCA7 Rabbit Polyclonal Antibody
TargetABCA7
ClonalityPolyclonal
Species/HostRabbit
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman
Predicted ReactivityMouse, Rat
Form/AppearanceLiquid
Concentration1mg/ml
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
ImmunogenKLH conjugated synthetic peptide derived from human ABCA7 (251-350/2146aa)
UniProt IDQ8IZY2
MW234 kDa
Tested applicationsFC
Dilution rangeFlow-Cyt=1ug/test
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesABC transporter ABCA7; ABC transporter member 7; A
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NoteFor research use only
ABCA7 Rabbit Polyclonal Antibody

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-ABCA7 antibody (orb1068), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 20% PBST for 20 min atroom temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

ABCA7 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ABCA7) Polyclonal Antibody, Unconjugated (orb1068) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

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