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AARE Rabbit Polyclonal Antibody

Catalog Number: orb100698

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb100698
CategoryAntibodies
DescriptionAARE Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IHC-Fr, IHC-P, WB
Predicted ReactivityBovine, Equine, Porcine, Rabbit, Rat, Sheep
ReactivityHuman, Mouse
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human AARE (101-200/732aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Form/AppearanceLiquid
ConjugationUnconjugated
MW81 kDa
TargetAPEH
UniProt IDP13798
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesAARE; ACPH; Acyl peptide hydrolase; Acylamino acid
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NoteFor research use only
Expiration Date12 months from date of receipt.
AARE Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (AARE) Polyclonal Antibody, Unconjugated (orb100698) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

AARE Rabbit Polyclonal Antibody

Sample: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-AARE (orb100698) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 81 kD, Observed band size: 78 kD.

AARE Rabbit Polyclonal Antibody

Sample: Lane 1: Mouse Kidney tissue lysates, Lane 2: Human U-2 OS cell lysates, Primary: Anti-AARE (orb100698) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 81 kDa, Observed band size: 76 kDa.

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