A-RAF antibody

• Catalog Number: orb555953
• Category: Antibodies
• Description: Rabbit polyclonal antibody to A-RAF
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human A-RAF. The exact sequence is proprietary.
• Concentration: 0.85 mg/ml
• Dilution range: WB: 1:500-1:3000 , IF: 1:100-1:1000 , IHC-P: 1:100-1:1000
• Form/Appearance: Liquid: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Purity: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• MW: 68
• Target: A-RAF
• UniProt ID: P10398
• Storage: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°lC or below. Avoid multiple freeze-thaw cycles.
• Buffer/Preservatives: 0.01% Thimerosal. 0.1M Tris-Glycine (pH7), 10% Glycerol
• Alternative names: A-Raf proto-oncogene, serine/threonine kinase , A-
• Note: For research use only
• Application notes: Positive control: HeLa , NIH-3T3
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of A431 cells using A-RAF antibody

Immunohistochemical staining of Cal27 Xenograft using A-RAF antibody

Western blot analysis of NIH-3T3 cells using A-RAF antibody

Western blot analysis of HeLa cells using A-RAF antibody