ARAF Antibody

• Catalog Number: orb546319
• Category: Antibodies
• Description: ARAF Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human ARAF recombinant protein (Position: E309-T413).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry, 1-3 μg/1x106 cells, Human Direct ELISA, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 68 kDa
• UniProt ID: P10398
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Serine/threonine-protein kinase A-Raf; Proto-oncog
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of SiHa cells using anti-ARAF antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of ARAF using anti-ARAF antibody.Lane 1:human HeLa cell; 2:human MCF-7 cell; 3:rat testis tissue;4:rat brain tissue; 5:mouse testis tissue; 6:mouse brain tissue.

IF analysis of ARAF using anti-ARAF antibody. ARAF was detected in an immunocytochemical section of MCF-7 cells.

IF analysis of ARAF using anti-ARAF antibody. ARAF was detected in an immunocytochemical section of PC-3 cells.