5HT7 Receptor Rabbit Polyclonal Antibody

• Catalog Number: orb155538
• Category: Antibodies
• Description: 5HT7 Receptor Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human 5HT7 Receptor/SR-7 (51-150/479aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 54 kDa
• Target: HTR7
• UniProt ID: P34969
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: SR-7; HT 7; 5 HT X; 5 hydroxytryptamine(serotonin)
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Suárez-Trujillo, A. et al. Short communication: Differences in distribution of serotonin receptor subtypes in the mammary gland of sheep, goats, and cows during lactation and involution J Dairy Sci, 102, 2703-2707 (2019)

PubMed: 30639027

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (5HT7 Receptor) Polyclonal Antibody, Unconjugated (orb155538) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (5HT7 Receptor) Polyclonal Antibody, Unconjugated (orb155538) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (5HT7 Receptor) Polyclonal Antibody, Unconjugated (orb155538) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Hippocampus (Mouse) Lysate at 40 ug, Hippocampus (Rat) Lysate at 40 ug, Primary: Anti-5HT7 Receptor (orb155538) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kD, Observed band size: 54 kD.

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Cerebellum tissue lysates, Lane 3: Rat Cerebrum tissue lysates, Lane 4: Rat Cerebellum tissue lysates, Primary: Anti-5HT7 Receptor (orb155538) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kDa, Observed band size: 50 kDa.