5HT7 Receptor Rabbit Polyclonal Antibody

SKU: orb155538

Description

5HT7 Receptor Rabbit Polyclonal Antibody

Research Area

Cell Biology, Obesity, Serotonin Receptors

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human 5HT7 Receptor/SR-7 (51-150/479aa)
• Target: HTR7
• Molecular Weight: 54 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

SR-7; HT 7; 5 HT X; 5 hydroxytryptamine(serotonin) receptor 7(adenylate cyclase coupled); 5 hydroxytryptamine(serotonin) receptor 7; 5 hydroxytryptamine receptor 7; 5-HT-7; 5-HT-X; 5-HT7; 5-hydroxytryptamine receptor 7; 5HT7; 5HT7R_HUMAN; HTR7; Serotonin 5 HT 7 receptor; Serotonin receptor 7.

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (5HT7 Receptor) Polyclonal Antibody, Unconjugated (orb155538) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (5HT7 Receptor) Polyclonal Antibody, Unconjugated (orb155538) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (5HT7 Receptor) Polyclonal Antibody, Unconjugated (orb155538) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Hippocampus (Mouse) Lysate at 40 ug, Hippocampus (Rat) Lysate at 40 ug, Primary: Anti-5HT7 Receptor (orb155538) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kD, Observed band size: 54 kD.

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Mouse Cerebellum tissue lysates, Lane 3: Rat Cerebrum tissue lysates, Lane 4: Rat Cerebellum tissue lysates, Primary: Anti-5HT7 Receptor (orb155538) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kDa, Observed band size: 50 kDa.

Quick Database Links

Gene Symbol

HTR7

UniProt

P34969

References

Suárez-Trujillo, A. et al. Short communication: Differences in distribution of serotonin receptor subtypes in the mammary gland of sheep, goats, and cows during lactation and involution J Dairy Sci, 102, 2703-2707 (2019)

PubMed: 30639027