5HT4 Receptor Rabbit Polyclonal Antibody

• Catalog Number: orb13228
• Category: Antibodies
• Description: 5HT4 Receptor Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Gallus
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human 5-HTR4 (21-120/388aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:200-800
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 43 kDa
• Target: HTR4
• UniProt ID: Q13639
• RRID: AB_10752375
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: 5-HTR4(5-hydroxytryptamine(serotonin) receptor 4a)
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (5HT4 Receptor) Polyclonal Antibody, Unconjugated (orb13228) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (orb868805) for 90 minutes, and DAPI for nuclei staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (5HT4 Receptor) Polyclonal Antibody, Unconjugated (orb13228) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (5HT4 Receptor) Polyclonal Antibody, Unconjugated (orb13228) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Bone (Mouse) Lysate at 40 ug, Primary: Anti-5HT4 Receptor at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43 kD, Observed band size: 43 kD.

Sample: Lane 1: Stomach (Mouse) Tissue Lysate at 40 ug, Lane 2: Cerebrum (Mouse) Tissue Lysate at 40 ug, Lane 3: Heart (Mouse) Tissue Lysate at 40 ug, Lane 4: Adrenal gland (Mouse) Tissue Lysate at 40 ug, Lane 5: Adrenal gland (Rat) Tissue Lysate at 40 ug, Primary: Anti-5HT4 Receptor (orb13228) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43 kD, Observed band size: 48 kD.

Sample: Large intestine (Mouse) Lysate at 40 ug, Primary: Anti-5HT4 Receptor (orb13228) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43 kD, Observed band size: 43 kD.