5HT2B Receptor Rabbit Polyclonal Antibody

SKU: orb155532

Description

5HT2B Receptor Rabbit Polyclonal Antibody

Research Area

Cell Biology, Obesity, Serotonin Receptors

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human 5HT2B Receptor/SR-2B (1-100/481aa)
• Target: HTR2B
• Molecular Weight: 54 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

SR-2B; 5 HT 2B; 5 HT 2B receptor; 5 HT(2B); 5 HT2B; 5 hydroxytryptamine(serotonin) receptor 2B; 5 hydroxytryptamine(serotonin) receptor 2B G protein coupled; 5 hydroxytryptamine 2B receptor; 5 hydroxytryptamine receptor 2B; 5-HT-2B; 5-HT2B; 5-hydroxytryptamine receptor 2B; 5HT(2B); 5HT2B_HUMAN; 5HT2F; Htr 2b; Htr2b; NP75 protein; SEROTONIN 5HT2B RECEPTOR; Serotonin receptor 2B; Stomach fundus serotonin receptor.

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (5HT2B Receptor) Polyclonal Antibody, Unconjugated (orb155532) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human HepG2 cell lysates, Lane 2: Human SH-SY5Y cell lysates, Lane 3: Human MCF-7 cell lysates, Lane 4: Human HeLa cell lysates, Primary: Anti-5HT2B Receptor (orb155532) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kDa, Observed band size: 60 kDa.

Quick Database Links

Gene Symbol

HTR2B

UniProt

P41595