5HT2B Receptor Rabbit Polyclonal Antibody

• Catalog Number: orb155532
• Category: Antibodies
• Description: 5HT2B Receptor Rabbit Polyclonal Antibody
• Target: HTR2B
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human 5HT2B Receptor/SR-2B (1-100/481aa)
• UniProt ID: P41595
• MW: 54 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SR-2B; 5 HT 2B; 5 HT 2B receptor; 5 HT(2B); 5 HT2B
• Note: For research use only

Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (5HT2B Receptor) Polyclonal Antibody, Unconjugated (orb155532) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human HepG2 cell lysates, Lane 2: Human SH-SY5Y cell lysates, Lane 3: Human MCF-7 cell lysates, Lane 4: Human HeLa cell lysates, Primary: Anti-5HT2B Receptor (orb155532) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kDa, Observed band size: 60 kDa.