5HT1D Receptor Rabbit Polyclonal Antibody

SKU: orb155528

Description

5HT1D Receptor Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse, Rat
• Predicted Reactivity: Human, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human 5HT1D Receptor/SR-1D (2-110/377aa)
• Target: HTR1D
• Molecular Weight: 42 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

SR-1D; 5 HT 1D alpha; 5 HT 1D; 5 HT1B; 5 HT1D; 5HT1D; 5 hydroxytryptamine(serotonin) receptor 1B; 5 hydroxytryptamine(serotonin) receptor 1D G protein coupled; 5 hydroxytryptamine 1B receptor(5 HT 1B)(Serotonin receptor)(5 HT 1D beta)(Serotonin 1D beta receptor)(S12); 5 hydroxytryptamine receptor 1D; HT1DA; HTR1D; HTR1D2; HTR1DA; HTRL; RDC4; S12; Serotonin receptor 1D; 5HT1D_HUMAN.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (5HT1D Receptor) Polyclonal Antibody, Unconjugated (orb155528) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Cerebrum tissue lysates, Lane 2: Rat Spinal cord tissue lysates, Primary: Anti-5HT1D Receptor (orb155528) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 42 kDa, Observed band size: 46 kDa.

Quick Database Links

Gene Symbol

HTR1D

UniProt

P28221