53BP1 Antibody

• Catalog Number: orb1530440
• Category: Antibodies
• Description: 53BP1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: between 350 and 400
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: 53BP1
• UniProt ID: Q12888
• NCBI: NP_005648.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: p53-binding protein 1; tumor suppressor p53-bindin
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human 53BP1 by western blot.Samples: Whole cell lysate from HeLa, HEK293T, and Jurkat cells. Antibody: Affinity purified rabbit anti-53BP1 antibody orb1530440 at 0.04 µg/ml.

Detection of mouse 53BP1 by western blot.Samples: Whole cell lysate from TCMK-1 and NIH 3T3 cells. Antibody: Affinity purified rabbit anti-53BP1 antibody orb1530440 at 0.04 µg/ml.

Detection of mouse 53BP1 by immunohistochemistry. Sample: FFPE sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-53BP1 used at a dilution of 1:1, 000 (1µg/ml).

Detection of human 53BP1 by immunohistochemistry. Sample: FFPE sections of human prostate carcinoma. Antibody: Affinity purified rabbit anti-53BP1 used at a dilution of 1:5, 000 (0.2 µg/ml).

Detection of human 53BP1 by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Affinity purified rabbit anti-53BP1 antibody.