53BP1/TP53BP1 Antibody

• Catalog Number: orb97102
• Category: Antibodies
• Description: 53BP1/TP53BP1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC, WB
• Predicted Reactivity: Hamster
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human 53BP1 (1955-1972aa ERIGFKQHPKYKHDYVSH), identical to the related mouse sequence.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, RatImmunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By HeatWestern blot, 0.1-0.5μg/ml, Human, Mouse, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 213574 MW
• UniProt ID: Q12888
• Sensitivity: > 5000 cells
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Tumor suppressor p53-binding protein 1;53BP1;p53-b
• Note: For research use only
• Application notes: WB: The detection limit for TP53BP1 is approximately 2.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Confocal immunofluoroscence analysis of A549 cells using 53BP1 antibody

Western blot analysis of Lane 1: 293T Cell Lysate, Lane 2: U20S Cell Lysate, Lane 3: HEPA Cell Lysate using 53BP1 antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded Human Intestinal Cancer Tissue using 53BP1 antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded Rat Brain Tissue using 53BP1 antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded Mouse Brain Tissue using 53BP1 antibody