53BP1 Antibody

• Catalog Number: orb1523957
• Category: Antibodies
• Description: 53BP1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: IHC, IP
• Reactivity: Human
• Immunogen: between 350 and 400
• Concentration: 1000 µg/ml
• Dilution range: WB - Not recommended; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: 53BP1
• UniProt ID: Q12888
• NCBI: NP_005648.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: p53-binding protein 1; tumor suppressor p53-bindin
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human 53BP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified goat anti-53BP1 antibody orb1523957 used for IP at 6 µg/mg lysate.

Detection of human 53BP1 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified goat anti-53BP1 (Cat. No. orb1523957) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB