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Catalog Number | orb345634 |
---|---|
Category | Antibodies |
Description | 42647 antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IP, WB |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Oct-4 protein. |
Concentration | 0.59 mg/mL |
Dilution range | ELISA: 1:350,000, IF: User Optimized, IP: User Optimized, WB: 1:1,000 - 1:1,500 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human and mouse Oct-4 protein. Cross-reactivity with Oct-4 from other species has not been tested. A BLAST analysis was used to suggest additional cross-reactivity with Oct-4 from chimpanzee, macaque and horse based on a 100% homology with the immunizing sequence; and mink, dog, bovine and pig based on a 93% homology. |
Conjugation | Unconjugated |
UniProt ID | Q01860 |
NCBI | 116235491 |
Storage | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-OCT4 antibody, OCT-4, OCT 4, Octamer b Read more... |
Note | For research use only |
Application notes | This affinity purified antibody has been tested for use in ELISA and western blotting. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of mouse embryonic fibroblast (MEF) cell lysate (lane 1) and HeLa nuclear extract (HN) (lane 2) using 42647 antibody
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