42647 antibody

• Catalog Number: orb345633
• Category: Antibodies
• Description: 42647 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IP, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Oct-4 protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:350,000, IF: User Optimized, IP: User Optimized, WB: 1:1,000 - 1:1,500
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human and mouse Oct-4 protein. Cross-reactivity with Oct-4 from other species has not been tested. A BLAST analysis was used to suggest additional cross-reactivity with Oct-4 from chimpanzee, macaque and horse based on a 100% homology with the immunizing sequence; and mink, dog, bovine and pig based on a 93% homology.
• Conjugation: Unconjugated
• UniProt ID: Q01860
• NCBI: 116235491
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-OCT4 antibody, OCT-4, OCT 4, Octamer b
• Note: For research use only
• Application notes: This affinity purified antibody has been tested for use in ELISA and western blotting.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of mouse embryonic fibroblast (MEF) cell lysate (lane 1) and HeLa nuclear extract (HN) (lane 2) using 42647 antibody