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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Mouse 4-1BB, His Tag at 0.5 ug/mL (100 ul/well),can bind Mouse 4-1BB Ligand , Fc Tag with a linear of 0.1-3 ng/mL. |
| Target | 4‑1BB Ligand |
| Tag | Fc Tag |
| MW | 49 kDa |
| Purity | >95% as determined by SDS-PAGE.Endotoxin level is less than 1.0 EU per ug by the LAL method. |
| Protein Sequence | Arg 104 - Glu 309 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH 7.5 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Human
Rabbit
Recombinant
Unconjugated
100 μl, 10 μlRecombinant Mouse 4-1BBL Protein [orb2994199]
Unconjugated
SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.
Predicted: 25.6 KDa. Observed: 35-40 KDa, reducing conditions
500 μg, 1 mg, 10 μg, 50 μgRecombinant Human 4-1BB Protein [orb2994312]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 44.2 KDa. Observed: 58 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human 4-1BB Protein [orb2994314]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 18.1 KDa. Observed: 28-35 KDa, reducing conditions
500 μg, 1 mg, 10 μg, 50 μgRecombinant Human 4-1BBL Protein [orb2994593]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 20.6 KDa. Observed: 16-20 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mg

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