4-1BB antibody

• Catalog Number: orb758985
• Category: Antibodies
• Description: Mouse monoclonal antibody to 4-1BB
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 4B4-1-1
• Tested applications: Blocking, ELISA, FC, IHC
• Reactivity: Human
• Isotype: IgG1
• Immunogen: The original antibody was generated by immunizing BALB/c mice with a fusion protein of human 4-1BB antigen and glutathione s transferase (GST).
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: 4-1BB
• UniProt ID: Q07011
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD137; MGC2172; Tumor necrosis factor receptor sup
• Note: For research use only
• Application notes: The antibody has good binding affinity for Con A-activated T cells and has an immunosuppressive activity towards lymphocyte proliferation in the in vitro mixed lymphocyte reaction. The binding of antibody to surface expressed 4-1BB molecule was assessed using flow cytometry and ELISA was used to check whether the cell culture had binding affinity to 4-1BB (Kim et al., 1995). The interaction of this antibody with CD137 is reported to induce an increase in TNF-α production by monocytes. This antibody competes with mAb M121 for binding to CD137 (PMID: 10607752). This antiboy also induces the production of IFN-γ in NK cells in presence of IL-15 (PMID: 20008791).This antibody can be used in blocking assays and staining methods (PMID: 14746806). Humanized versions of this antibody (Hz4B4-1) and (Hz4B4-2) showed a 1.5 fold and 7.3 fold increase and in binding affinity for 4-1BB as compared to their parental mouse antibody. Additionally, H4B4 mediated antibody-dependent cellular toxicity of activated human peripheral blood T cells and CEM cells in a dose dependent manner (PMID: 11186149). Humanized version of this antibody is available on request.
• Expiration Date: 12 months from date of receipt.