CD137 Antibody

• Catalog Number: orb1519872
• Category: Antibodies
• Description: CD137 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: FC, ICC, IHC, IP, WB
• Reactivity: Human
• Immunogen: residues 24-189 (ECD)
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1000; IP - 20 µl/1 mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: CD137
• UniProt ID: Q07011
• NCBI: NP_001552.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Alternative names: ILA; interleukin-activated receptor, homolog of mo
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human CD137 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, HDLM-2, My-La CD4+, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD137 recombinant monoclonal antibody (orb1519872) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human CD137 in FFPE tonsil by IHC. Antibody: Rabbit anti-CD137 recombinant monoclonal antibody (orb1519872). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Detection of human CD137 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HDLM-2 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD137 recombinant monoclonal antibody (orb1519872) used for IP at 6 µl/mg lysate. CD137 was also immunoprecipitated by a previous lot of this antibody (orb1519872). For blotting immunoprecipitated CD137, orb1519872 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human CD137 in FFPE HDLM-2 by ICC. Antibody: Rabbit anti-CD137 recombinant monoclonal antibody (orb1519872). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.