3-Nitrotyrosine Rabbit Polyclonal Antibody

• Catalog Number: orb1532
• Category: Antibodies
• Description: 3-Nitrotyrosine Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Mouse
• Reactivity: All
• CAS Number: 621-44-3
• Isotype: IgG
• Immunogen: KLH conjugated Nitrotyrosine
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:50-200, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: NO tyrosine; nTyr; Nitrotyrosine; nitro-Tyrosine;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: Hela (blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG (orange), Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue), Dilution: 1:100 in 1X PBS containing 0.5% BSA, Primary Antibody Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA (green).

Sample: 3-Nitrotyrosine-BSA conjugate Protein, Primary:Anti-3-Nitrotyrosine (orb1532) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Nitro tyrosine Polyclonal Antibody, Unconjugated (orb1532) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.