14-3-3 zeta+delta Rabbit Polyclonal Antibody

SKU: orb100007

Description

14-3-3 zeta+delta Rabbit Polyclonal Antibody

Research Area

Alzheimer's Disease, Cancer Research, Cell Biology, Cytoplasmic, Neurology, Neuroscience, Signal Transduction, Synapse Markers, Tumor Biology

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB=1:500-2000, Flow-Cyt=1ug/Test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human 14-3-3 zeta/delta (51-150/245aa)
• Target: YWHAZ
• Molecular Weight: 27 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

14 3 3 protein delta; 14 3 3 protein zeta/delta; 14 3 3 protein/cytosolic phospholipase A2; 14-3-3 protein zeta/delta; 14-3-3zeta; 14-3-3 delta; 14-3-3 zeta; 14-3-3delta; 1433Z_HUMAN; KCIP 1; KCIP-1; KCIP1; MGC111427; MGC126532; MGC13815; phospholipase A2; Protein kinase C inhibitor protein 1; Tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein zeta polypeptide; YWHAZ.

Blank control (black line): Raji (black) (The cells were fixed with 2% paraformaldehyde (10 min), then permeabilized with PBST for 30 min on room temperature), Primary Antibody (rad line): Rabbit Anti-14-3-3 zeta+delta antibody (orb100007), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (grenn line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 µg/test.

Blank control (blue line): HL60 (fixed with 70% ethanol (overninght at 4℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-14-3-3 zeta+delta antibody (orb100007), Dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-14-3-3 zeta+delta (orb100007) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 27 kD, Observed band size: 27 kD.

Sample: Cerebrum (Rat) Lysate at 40 ug, Primary: Anti-14-3-3 zeta+delta (orb100007) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 27 kD, Observed band size: 27 kD.

Quick Database Links

Gene Symbol

YWHAZ

UniProt

P31946