14-3-3 theta Antibody

• Catalog Number: orb1525379
• Category: Antibodies
• Description: 14-3-3 theta Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP
• Reactivity: Human
• Immunogen: between 100 and 150
• Concentration: 1000 µg/ml
• Dilution range: WB - Not recommended; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: 14-3-3 theta
• UniProt ID: P27348
• NCBI: NP_006817.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: HS1; tyrosine 3-monooxygenase/tryptophan 5-monooxy
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human 14-3-3-theta by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-14-3-3-theta antibody orb1525379 used for IP at 6 µg/mg lysate.

Detection of human 14-3-3-theta by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-14-3-3-theta (Cat. No. orb1525379) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB