14-3-3 Theta/Tau (phospho-Ser232) antibody

• Catalog Number: orb769496
• Category: Antibodies
• Description: Rabbit polyclonal antibody to 14-3-3 Theta/Tau (phospho-Ser232)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human 14-3-3 thet/tau around the phosphorylation site of Ser232. AA range:196-245
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• MW: 28
• Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-YWHAQ antibody, anti-14-3-3 protein theta ant
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of Hela cell. 1, 14-3-3 θ/τ (phospho Ser232) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min).

Western blot analysis of 293T MOUSE-BRAIN using p-14-3-3 θ/τ (S232) antibody. Antibody was diluted at 1:500.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using 14-3-3 thet/tau (Phospho-Ser232) Antibody.

Immunofluorescence analysis of HeLa cells, using 14-3-3 thet/tau (Phospho-Ser232) Antibody. The picture on the right is blocked with the phospho peptide.