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Catalog Number | orb1528031 |
---|---|
Category | Antibodies |
Description | 14-3-3 Sigma Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human |
Immunogen | between 198 and 248 |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate; IHC - 1:2,000 to 1:10,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | 14-3-3 Sigma |
Hazard Information | Non-Toxic |
UniProt ID | P31947 |
NCBI | NP_006133.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | epithelial cell marker protein 1; Stratifin; 14-3- Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human 14-3-3 Sigma by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, A-549, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-14-3-3 Sigma antibody orb1528031 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human 14-3-3 Sigma by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-14-3-3 Sigma antibody orb1528031.
Detection of human 14-3-3 Sigma by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-14-3-3 Sigma (Cat. No. orb1528031) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB
ELISA, FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Hamster | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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